western blot quantification imagej and ccd imager

Western blot quantification imagej and ccd imager install#
Western blot quantification imagej and ccd imager upgrade#

This background can come from non-specific binding during antibody incubation, membrane background in the case of fluorescent images, or background arising from the instrumentation itself.įor bright, isolated bands on smooth backgrounds, this process can be relatively straightforward. No western blot is perfect, and all blot images show some level of background. Therefore, band quantity is often referred to as band 'volume' by imaging software. Each pixel also carries signal intensity information, which can be thought of as data in the third dimension. The pixels that comprise the part of a band have x and y coordinates, so outline an area. Therefore, band quantity can be thought of as a volume, which is the total amount of signal for all the pixels within that band. Each pixel in a blot image has an x and y coordinate, in addition to an intensity value, which occupies the third dimension. A digital image of a blot can be thought of as data in three dimensions. A western blot image is made up of pixels, which contain information about how much signal was collected at each location in the image. Band VolumeĪ protein band is a feature that appears in a western blot image. Note: western blots are semi-quantitative so they are ideal for showing relative protein expression rather than an absolute quantity. By analyzing the intensity of the signal, you may determine whether the expression of the target protein in one sample has increased or decreased relative to another sample or control. The signal intensity of the band is directly proportional to the concentration of your target protein. Quantitating a western blot refers to the measurement of the signal emitted by your protein band(s) of interest.

Western blot quantification imagej and ccd imager upgrade#

These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit ( 12003263).Blot Quantitation and Background What Does Quantitation Mean? Note: Also available are the ChemiDoc Imaging System (12003153), which provides a more limited fluorescence capability than ChemiDoc MP Systems. UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems ( 12003914).Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems ( 12003915).Holder for ChemiDoc Sample Trays and UV Shield ( 1708377).Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain ( 12003027).White Sample Tray for Coomassie Blue, copper, silver, and zinc stains ( 12003026).Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby ( 12003028).

Western blot quantification imagej and ccd imager install#

Image Lab Software to install on your computer.

Internal computer with Image Lab Touch Software.

with fluorescence, chemiluminescence, or colorimetric detection

Blotting - identify and quantify proteins, nucleic acids, protein-ligand interactions, etc.

Electrophoresis - protein, including stain-free, and nucleic acid gel imaging and documentation.

Performing Stain-Free western blotting using the ChemiDoc MP System provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly

Sample Trays and Smart Tray Technology - three sample trays available to cover diverse imaging applications.

Stain-free protein normalization - stain-free imaging permits the normalization of bands to total protein in both in gels and blots eliminates the need for housekeeping proteins.

Ease of use - features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images.

Multiplex fluorescent western blotting - detect up to three proteins simultaneously and eliminate the need for stripping and reprobing.

Improve your imaging capabilities with the ChemiDoc MP - benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright Fluorophores see the fluorophore compatibility chart.

All-in-one flexible imaging - get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system.

Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.